C19 plasmid? 5) Which of the following is FALSE eganding All of the shove are fo
ID: 272335 • Letter: C
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C19 plasmid? 5) Which of the following is FALSE eganding All of the shove are folowing would be true? into the 57) If pUC19 as shown above is used to transform E coli, which of the E woll. which of the tolke oylinker. The transformation wouldn't work unless a gene is polylinker. a) cells would be unable to grow on agar containing ampicillin c) The transformed cells would grow white on agar containing d) The transformed cells would grow blue on agar containing amplciin illin and X-gal. 58) Which of the following has NOT been produced by transgenic E. colr a) human growth hormone b) clotting factor c) insulin d) red blood cells e) all of these have been produced by transgenic E. coli 59) In the PCR lab, DNA polymerase from Thermus aquaticus was used to perform which step of the c) restriction cuts PCR process? a)denaturation b) extension d) annealing e) staining 60) In the gel below, what is the approximate size of the DNA fragment highlighted by the arrow? a)411bp b)725 bp c) 1115 bp d) 4120bp e) cannotbe determined 1 2 34 56 9416 bp 23110 bp 3000 bp 2027 bp 25 bo 70 bpExplanation / Answer
56. all of the above are true regarding pUC19(IN pUC19 gene insert at polylinker site, due to disturbance of lac z gene it stop production beta galactocidase and colony become white, and amp resistance is the marker gene in pUC19.)
57. the transformed cells would grow white on agar containing amp. And X-gal( because gene insert lac-Z gene and stop beta galactocidase production which turns X-gal blue( transformation would be work in insert anywhere in plasmid but polylinker site is suitable for insertion and identification, amp resistance gene is not disturb so cell can grow on amp containg media,)
58. red blood cell can not produce by transgenic E.coli( human growth factor, clotting factor and insulin are protein and gene can be inserted in E.coli but RBC sare whole cell it can not produce).
59.In PCR lab, DNA polymerase is used for extension because ( the polymerase enzyme add nucleotide to 3’ end ( denaturation is done by high temperature or chemicals, restriction cuts done by restriction enzymes like EcoR1, annealing is attachment of primer and staining is done by dye like EtBr.)
60) the size of DNA band can not be determine because it is very close to both marker size .
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