The image shows the enzyme activity of pepsin and trypsin as a function of pH, p
ID: 272361 • Letter: T
Question
The image shows the enzyme activity of pepsin and trypsin as a function of pH, protein enzymes that hydrolyze peptide bonds. A scientist hypothesizes that aggregation of pepsin and trypsin at high and low pH is the reason for decreasing enzyme activity.
QUESTIONS:
1. Does the data strongly support thte scientists hypothesis? Yes or no?
2. How strongly does the data support the scientists hypothesis? Explain.
3. Give two other explanations for the change in enzymatic actiivty as a function of pH.
pepsin trypsin 1 2 3 4 5 6 7 8 9 10 11 12 pHExplanation / Answer
(1)Yes, data strongly support the scientist's hypothesis
(2)We could see that these two enzymes have bell curves at different pH range, showing their optimal activity at different pH. Optimal pH for pepsin is 3 and for trypsin 8. This is a strong evidence for differing enzyme activity at high and low pH for the respective enzyme,
(3)The most favourable pH value where the enzyme activity is highest is known as the optimum pH. Low pH tends to protonate and high pH tends to deprotonate the active site amino acids and substrates causing loss of activity. Extreme pH changes can cause denaturation of enzymes resulting in total loss of their activity
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