4) Consider the following sequence of a DNA double helix. (20 points) 2 A) Using

Question

4) Consider the following sequence of a DNA double helix. (20 points) 2 A) Using the genetic code table below, A) determine the mRNA sequence resulting from transcription. (5 points) Phe UCU Tyr UGU Ser UAC UGCCys UAA Stop UGA Stop A UUGLeUCGUAG Stop UGG Trp G cUU CUC Leu CUA CCA Pro Arg A AUC?lle ACC AGC Ser Thr AAA AUG Met ACGAr AAG J Lys AGG Arg GCU ASp GGC GUC G GUA Val GCA Gly a combination of intracellular and extracellular conditions, the mRNA obtained in part (A) is used to either tein I) or an l1 amino acid protein (Protein II). What are the sequences of these two produce a 20 amino acid protein (Prot proteins? (5 points)

Explanation / Answer

DNA: 5’CAAATGCGACGCGGGCCGTCATGCTGTTGGCTTTCCGTACTCGTGAGACACACTAGGAAGATTTAGGTT 3’

A)

mRNA: 5’CAAAUGCGACGCGGGCCGUCAUGCUGUUGGCUUUCCGUACUCGUGAGACACACUAGGAAGAUUUAGGUU 3’

B)

Protein I: Met-Arg-Arg-Gly-Pro-Ser-Cys-Cys-Trp-Leu-Ser-Val-Leu-Val-Arg-His-Thr-Arg-Lys-Ile-Stop (20 amino acids)

Protein II: Leu-Ser-Val-Leu-Val-Arg-His-Thr-Arg-Lys-Ile-Stop (11 amino acids)

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