14. Match the following molecules with its class of macromolecule: A. Proteirn B

Question

14. Match the following molecules with its class of macromolecule: A. Proteirn B. Lipid C. Nucleic Acid D. Carbohydrate Keratin Cellulose DNA cholesterol glycogen collagen elastase enzyme RNA -?myoglobin gene sphingomyelin collagen 15. Christian Anfinsen obtained a quite different result when reduced ribonuclease was re-oxidized while it was still in 8M urea and the preparation was then dialyzed to remove the urea. Ribonuclease re-oxidized in this way had only 1% of the enzymatic activity of the native protein. Why were the outcomes so diffefent when reduced ribonuclease was re-oxidized in the presence and absence of urea? (see article on Blackboard) 16. What is the pH of a solution of 0.8M acetic acid and 0.5M sodium acetate? pKa of acetic acid 4.76? (show your work)

Explanation / Answer

14. Match the following molecules with its class of macromolecule:

A. Protein : Keratin, collagen, elastase, enzyme, myoglobin, Collagen

B. Lipid: cholesterol, sphingomyelin

C. Nucleic Acid : DNA, RNA, gene

D. Carbohydrate : Cellulose, glycogen


15. Christian Anfinsen obtained a quite different result when reduced ribonuclease was reoxidized while it was still in 8 M urea and the preparation was then dialyzed to remove the urea. Ribonuclease reoxidized in this way had only 1% of the enzymatic activity of the native protein. The outcomes were so different when reduced ribonuclease was reoxidized in the presence and absence of urea because wrong disulfides formed pairs in urea. There are 105 different ways of pairing eight cysteine molecules to form four disulfides and only one of these combinations is enzymatically active. The 104 wrong pairings called as scrambled ribonuclease.

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