3. Suppose that thermodynamic parameters for the folding of an enzyme under a ce

Question

3. Suppose that thermodynamic parameters for the folding of an enzyme under a certain set of solution conditions at 298 K were as follows. AG'folding--10 kJ/mol; AS.folding-0.05 kJ/K.mol. The folding process is as follows: unfolded protein folded proteirn A. What is AH folding? Show your work B. Under these solution conditions, at 298 K, this enzyme would be mainly in (1) the folded state. (2) the unfolded state. (3) neither. Select the best answer and briefly EXPLAIN your answer on the basis of the information given above. C. Select the best answer. The overall entropy change (AS' folding) under these conditions (1) favors folding. (2) favors unfolding. (3) favors neither folding nor unfolding. D. Which component of the entropy change must predominate in folding of this protein? (1) AS conformational (chain entropy). (2) AShydrophobic (the hydrophobic effect). (3) Neither. Select the best answer and briefly EXPLAIN your answer in terms of the change in conformational entropy and the entropy change due to the hydrophobic effect when a protein folds E. Select the best answer. What type of interactions between the two a helices are most important in stabilizing coiled coil structures like the one shown in this protein? (1) hydrogen bonds. (2) hydrophobic interactions. 3) ionic interactions F. Select the best answer. For the structure shown above to form the kinds of amyloid structures typical of many diseases involving misfolding of proteins, the structure would have to (1) bind to other ? helical coiled coil structures into a 4-helix structure, then 8 helices, 16, etc., to form a multi- helix aggregate (2) rearrange the helices into left-handed a helices prior to aggregating into a multi-helix aggregate as above. (3) rearrange into more ß secondary structure, to form aggregates of stacked "cross P" sheets (4) form aß barrel structures that stack onto each other to form fibrils made up of long tubules

Explanation / Answer

3. A. G = H - T x S

So H = G + T. S

H = 10 + 298 x 0.05

H = 10 + 14.9

H = 24.9 KJ/mol

E. Answer is 3. Ionic interactions. Due to charge groups at periphery

F. Answer is 1

C. Answer is 2

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