1. Traditional sequencing of genomes was long and5. tedious because: Oxford Nano
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1. Traditional sequencing of genomes was long and5. tedious because: Oxford Nanopore NGS includes all the following characteristics, except: Genomic DNA fragments were cloned into plasmids to generate a genomic DNA library Plasmids were individually sequenced using Sanger sequencing Complicated computational methods had to performed to identify overlapping pieces a. a. It has the pore size of a 10 DNA molecules b. A DNA Polymerase molecule linked to the b. opening of the pore c. The protein alpha-hemolysin forming a pore d. Current change depending on the sequence c. d. All of the above 6. A Contig is described as which of the following: An assembly of small DNA sequences into a long contiguous sequence a semiconductor chip a type of fluorescent nucleotide none of the above a. Compared to Sanger sequencing, Next Generation Sequencing (NGS) has: 2. b. c. d. a. Lower cost per nucleotide for sequencing b. High paralelization of sequencing c. d. High throughput of sequencing All of the above 7. Humans have selectively bred crops and livestock to propagate desirable traits in a process termed: The following describe a flow cell used in NGS, except a. Natural selection b. Artificial selection c. Genetic engineering d. None of the above 3. It undergoes successive rounds of flooding/washing with a fluorescent nucleotide The surface is covered with short RNA oligonucleotides 8. GMO stands for: a. Genetically Modified Oranges b. Good Modified Organisms c. Genetically Modified Organism d. None of the above c. After each cycle, fluorescent signals are measured on the flow cell Specific locations of fluorescence are tracked to indicate the sequence at each registered point on the flow cell d. 9. GloFish are an example of a. Natural selection b. Genetic engineering c. Artificial Selection 4. lon torrent is a type of NGS that measures nucleotide incorporation based on which change: None of the a a. pH b. temperature c. sound d. none of the above 10. Chromatography can be a method of separation a. Size b. Charge C. AffinityExplanation / Answer
Answer 1. The correct option is D. All of the above.
Answer 2. The correct option is D. All of the above. NGS costs .5$ per reaction compared to 500$ for sanger sequencing per 1000 bp. In NGS several reactions can be performed simultaneously with higher throughput capacity compared to that of sanger sequencing.
Answer 3. The correct option is B. Its not RNA it is DNA oligonucleotides that are used to cover the surface.
Answer 4. The correct option is A. pH. Ion Torrent acts as apH meter to identify the nucleotide incorporation.
Answer 5. The correct option is A. It is not the pore size of 10 DNA molecules but a single DNA molecule.
Answer 6. The correct option is A. This is the definition for a contig.
Answer 7. The correct option is B. Artificial selection. Selective breeding is also known as artificial selection.
Answer 8. The correct option is C. Genetically modified organisms.
Answer 9. The correct option is B. Genetic engineering. The GloFish is a patented and trademarked brand of genetically engineered fluorescent fish.
Answer 10. Chromatography are of different types and it can seperate mixtures on the basis of size (size exclusion chromatography), charge (ion exchange chromatography) and as well as affinity (affinity chromatography). So the answer should be "All of the above" which i guess is the option "D" but not shown here.
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