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Question

le Edit Vew History Bookmarks People Window Help fle ll 17%20(4).pdf Here's three strands of aligned DNA from Homo trumpensis: 1. CGC CTA TAC GCG TCT ACG ATG TAA CTC TGT TGA 2. CGC CTA TAC GCG TCT AGG ATG TAT CTC TGT TGA 3. GGC CTA TAC GCG TCT AGG ATG TAT CTC CTC CTC Do they look like genes? Do they have ORFs? Can you identify where TBP would bind? One contains a NONSENSE mutation; identify it. One contains a microsat; identify it. Of the three organisms containing this region, only one is disease-free; which one do you think? (How many codons?) Identify SNPs. Make a cladogram based on relatedness; now turn it into a Newick file.

Explanation / Answer

A) Yes, they look like genes with TATA box, start codon, ORF and stop codon. Open reading frame of a gene is the sequence where the sense strand is read.

1. CGC CTA TAC GCG TCT ACG ATG TAA CTC TGT TGA

2. CGC CTA TAC GCG TCT AGG ATG TAT CTC TGT TGA

3. GGC CTA TAC GCG TCT AGG ATG TAT CTC CTC CTC

B) TATA binding protein is a transcription factor binds specfically to a DNA sequence called TATA box.

1. CGC CTA TAC GCG TCT ACG ATG TAA CTC TGT TGA

2. CGC CTA TAC GCG TCT AGG ATG TAT CTC TGT TGA

3. GGC CTA TAC GCG TCT AGG ATG TAT CTC CTC CTC

C) Nonsence mutation is a genetic mutation in a DNA sequence that results in a shorter unfinished protein product. It is in 1st sequence UGG leads to stop codon

1. CGC CTA TAC GCG TCT ACG ATG TAA CTC TGT TGA

CGC CUA UAC GCG UCU ACG AUG UAA CUC UGU UGA

UAA is a stop codon

d) A microsatellite is a tract of repetitive DNA in which certain DNA motifs are repeated. In this case 3rd sequence has microsat with CTC sequence.

3. GGC CTA TAC GCG TCT AGG ATG TAT CTC CTC CTC

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