Chapter 6 Concepts and Question Review Name abescl merncz 1. Which media makes i

Question

Chapter 6 Concepts and Question Review Name abescl merncz 1. Which media makes it easy to distinguish colonies of different microbes on the same plate 2. What is an example of a differential media? 3. What does hemolysis mean? 4. Which type of media suppresses unwanted microbes and encouragesd S. What is an example of a selective media? 6. What is an example of a media that is both selective and differential? 7. Give an example ofwhen above media may be useful. &. How many species does a pure culture contain? 9. What is a colony? 10. What does CFU stand for? 11. What are the 4 ways prokaryotes reproduce? esired microbes? ?.3. d. 4 12. What steps occur during binary fission? 13. What is generation time? 14. Why are logarithmic scales used to represent population growthversus exponential growth? 15. What is the formula to calculate the size of a population over-time? 16. Ifyou have 500 bacteria with a doubling time of 20 minutes, how many cells would there be in three hours (assume optimal growth conditions)?

Explanation / Answer

Question 1

The answer is Differential media

it is the media used to separate or distinguish colonies of different organisms.

Question 2

Answer

The examples of differential media are as follows

i. MacConkey agar – more selective differentiation of enteric bacteria

ii. Blood agar – selective for hemolytic bacteria

iii. Eosin-methylene blue agar

Question 3

Answer

The destruction of lysis of red blood cells is called hemolysis. which is used to observe the hemolytic reaction in differential media. they are three types of Alpha hemolysis, beta hemolysis, and gamma hemolysis.

Question 4

Answer

Selective Media which is used to suppress the growth of unwanted microbe and encourage the growth of desired microbes.

Question 5

Answer

the examples of selective media are

i. thayer martin agar

ii. Mannitol salt agar

iii. Crystal violet blood agar

iv. Pseudosel agar

v. Lowenstein Jensen media and Mac Conkey agar

Question 6.

The agar which acts both selective and differential media are Blood agar, Mac Conkey agar, Mannitol salt agar.

Question 7

Answer

Blood agar media used to separate different hemolytic bacteria that is alpha hemolytic, beta-hemolytic and gamma hemolytic bacteria.

Question 8

Answer

the pure culture contains only one species and it can classify and identify the bacteria.

Question 9

Answer

The colony is the group of cells enough to see with the naked eye without the use of a microscope

Question 10

Answer

Colony Forming Units.which is used to express the number of bacterial cells in the group.

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