Later this week the concentration of your DNA extraction will be quantified usin

Question

Later this week the concentration of your DNA extraction will be quantified using a UV spectrophotometer by measuring its absorbance in units of optical density (OD). DNA absorbs light most strongly at the ultraviolet wavelength of 260 nm. The absorbance of double stranded DNA (dsDNA) at 260 nm (A260) is used to estimate concentration, with 1.0 OD equal to a dsDNA concentration of 50 g/ml. Using this information we can calculate the concentration of dsDNA in our extractions using the following formula: 5. dsDNA concentration 50 ?g/ml x 0D260 x dilution factor

Explanation / Answer

Answer-

dsDNA concentration = 50 ?g/mL × OD260 × dilution factor

dsDNA concentration = 50 ?g/mL × 0.65 × 20

dsDNA concentration = 650 ?g /mL or 0.650 mg/mL

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