You isolate chromatin from mouse liver cells (hepatocytes) 1. You set aside one

Question

You isolate chromatin from mouse liver cells (hepatocytes) 1. You set aside one sample and isolate DNA from it - removing all traces of protein. 2. You treat another 5 samples (equal amounts) of the chromatin with increasing amounts of DNAsel enzyme. 3. After you inactivate the DNAsel in these samples, you isolate the DNA from each (again, removing all traces of protein) 4. Now you cut all 6 of your isolated DNA samples with the restriction endonuclease EcoRI. 5. You run all 6 samples on an agarose gel (to separate the DNA fragments according to size). 6. You do a Southern Blot, and hybridize the nylon sheet with a DNA probe that you know is homologous to a region near an EcoRl site. These are your results: 2kb

Explanation / Answer

In the mouse genome, there is a 9kb EcoRI fragment that is homologous to your probe.

This can be explained on the basis that if our probe was homologous for the entire 9kb fragment, we would not expect the 2kb banding pattern. This is because the DNAaseI treatment will likely disrupt the homologous region.

We can say that there is a 2kb fragment in the 9kb fragment that is homologous to our probe. So in both cases with and without treatment the probe will hybridize the 2kb fragment. With increasing concentration of DNAaseI it is likely to get more of the 2kb fragment.

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