Transler of Bacteria Aseptic Technique Nutrient Semisolid Agar Deep Show the loc
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Transler of Bacteria Aseptic Technique Nutrient Semisolid Agar Deep Show the location of bacterial growth and note any P igmene formation. Lactococeus lactis Comparison of Broth and Slant Cultures Slans Culure Brech Caleure Gram sain Morphology Questions 1. Did growth occur at different levels in the agar deep? 2. Were any of the bacteria growing in the semisolid agar deeps motile? Explain. 3. What other methods can be used to determine motiliry? 4. What is the primary use of slants? Of deeps? Of broths?Explanation / Answer
The Lactococcus lactis in a nutrient agar slant- beaded
The pattern growth of the Pseudomonas aeruginosa in a nutrient agar slant-spreading
The growth pattern of the Proteus vulgaris in a nutrient agar slant- Echibulate.
1. Yes, growth did occur at different levels in the agar deep.
2.Yes; you can tell the bacteria was motile by the way it was spread in the agar deeps. It spread from the point of inoculation. The extent to which the bacteria moved is determined by the distance of spread from the inoculation.
3. Methods to measure tha motility
1. Wet Mount slide
When working with nonpathogens, the simplest way to determine motility is to place a few loopfuls of the organism on a clean slide and cover it with a cover glass. In addition to being able to determine the presence or absence of motility, this method is useful in determining cellular shape (rod, coccus, or spiral) and arrangement (irregular clusters, packets, pairs, or long chains).
2. Hanging Drop slide
Hanging drop slides are useful in observing the general shape of living bacteria and the arrangement of bacterial cells when they associate together. Organisms are observed in a drop that is suspended under a cover glass in a concave depression slide. The slide for a hanging drop is ground with a concave well in the center; the cover glass holds a drop of the suspension. When the cover glass is inverted over the well of the slide, the drop hangs from the glass in the hollow concavity of the slide. Since the drop lies within an enclosed glass chamber, drying out occurs very slowly. A ring of Vaseline around the edge of the cover slip keeps the slide from drying out.
4. the primary use of
Slants-A solid growth site that is easier to store/transport.
Deeps-Used to grow bacteria that needs less oxygen
Broths-To keep a large number of bacteria in a small space.
5.We can determine whether or not the broth cultue is pure , only by agar slant cultures. Simple micrscopy and agar deep culture fails to do so.
6.Loops are preferable when you use bacteria slants and petri dishes
A needle is preferable for transferring bacteria Into a deep culture.
7.The purpose before is to kill any previous bacteria on the loop before it is exposed to the preferred bacteria; the purpose after is to kill off the bacteria it has been exposed to.
8.It must be cool because if it does not the heat will kill the bacteria in the culture. No, you should not set the loop down to let it cool. It will not be a red color when it will cool; it will go back to the black color.
9.Aseptic techniques are important because it Involves certain procedures that have to be performed carefully in order to minimize contamination by pathogens.
CRITICAL THINKING
1.The broth culture allows a lot more bacteria to grow within a smaller area, while there is little room for bacteria to grown on a slant culture.
2.By allowing for oxygen-loving bacteria to float to the top, forming a colony. It increases the chance of its survival.
3.You could tell if the media were sterile by comparing it to the control.
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