There is a protein (or technique) widely used in biologicalresearch and win 2008

Question

There is a protein (or technique) widely used in biologicalresearch and win 2008 Nobel

Prize in Chemistry. Please specify what is it?

(Let’s call it X protein now because I have to mentionthis protein in the following question)

What is the application of this protein/ technique to sciencelabs?

You know recombinant DNA is very powerful in genetics study, andthe genetic technique is commonly use in C. elegans. Nowyou plan to study gene ysh-1(hypothetical) of C.elegans. You want to know when ysh-1(or YSH-1protein) is expressed.

How do you apply the technique (X protein) in this study?

What is the disadvantage of this technique (hint: ifysh-1is only expressed in larva worm, whatmisunderstanding you might have in applying this technique)?

And how do you plan to solve this problem?

Explanation / Answer

I think you are looking for GFP(Green Fluorescent Protein). It basically glows under UV light so you can track steps in the process of things like DNA recombination. In DNA recombination the host bacteria(receiving the vector plasmid) are often given an extra gene to produce resistance for  a specific illness/disease. This way, all of the bacteria which did not actually take up the new plasmid vector, will also not have the resistor gene. Well, you can do the exact same thing with GFP protein so that instead of killing the unsuccesful bacteria, you can cause the successful ones to glow and see if the host bacteria are accepting the gene. So, to apply it to that study, you simply pair the GFP protein in the same plasmid as the gene of interest. If they glow, the gene is expressed.

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