Use the following diagram to answer questions 42-43. The following diagram hih h

Question

Use the following diagram to answer questions 42-43. The following diagram hih has two eons,restriction encyme sites, and a cmenmtary próbe as labeled shows the genomic locus for P K 2.5 2 Exon 2 probe P Pstl site K Kpnl sitee Numbers indicate restriction fragment size in kilobases Exon 1 -0.8 kb Exon 2 3.7 kb 42. What size band(s) would you expect to see in Southern blots using the diagrammed probe and enzymes listed below? a. Southern (Pstl digest) 1 kb and 3 kb b. Southern (Pstl digest) 4.5 kb only c. Southern (Kpnl digest) 1.5 kb, 4 kb, and 5 kb d. Southern (KpnI digest) 5 kb only e. Southern (Kpnl/PstI double digest) 1 kb and 3 kb 43. What size band(s) do you expect to see in a Northern blot? a. 3 kb b. 3.7 kb c. 4.5 kb d. 10.5 kb e. 0.8 kb and 3.7 kb

Explanation / Answer

42. The only segment that will be detected will be one with the probe as that is the principle of southern blotting. The correct answer is d. After digestion with KpnI three segments will be created of size 4,5, 1.5 KB

43. Northern blot detects rna, and RNA only contains exons and this case Exon with a probe will be a 3 kB segment containing the probe

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