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L DETERMINING GENE ORDER We want to map the distance between genes A (green colo

ID: 275820 • Letter: L

Question

L DETERMINING GENE ORDER We want to map the distance between genes A (green color), B (rough leaf), and C(normal fertility). Each genes has a recessive allele (a- yellow, b glossy and cvarable). Your testcross genotypes (use slash notation) will be Note: The actual configuration of the trihybrid will be deduced as part of the process x (tester) Table 1 . Test-cross progeny phenotypes #s: 2) Yellow, rough, normal: 50 5) Green, glossy, normal :700 8) Yellow, glossy, variable: 95 3) Green, rough, variable:5 6) Yellow, glossy, normal: 5 1) Green, rough, normal: 95 4) Yellow, rough, variable: 700 7) Green, glossy, variable: S0 Based on these results, are the genes linked orenot? Based on the results above, the parental progeny can be observed in the phenotypes #s: and - and their genotypes (use slash notation) are: and The actual configuration of the parental chromosomes in the trihybrid plant is (use slash notation) The double crossover progeny phenotypes #s are: notation) are: Compare the double crossover dasses with the parental dlasses. Based on this comparison, the actual gene order is: Complete the figure (genes are located on the long arm of the chromosomes) and and their genotypes (use slash and Trihybrid parental chromosomes Double crossover recombi For the remaining questions ignore the male gamete when writing the genotypes Rewrite the genotype of the mating results from Table 1 with the genes in the correct order: Il. MAP DISTANCE To figure out the distance, we must identify the single crossover classes (write as genotypes) The single crossover classes are ldentify the offspring classes that have recombined between the A and B genes. These are the single as well as the double crossovers and Distance between A and B- ldentify the offspring classes that have recombined between the A and Cgenes. These are the single as well as the double crossovers and Distance between A and C Distance between B and C FINAL MAP:

Explanation / Answer

Test cross genotypes would be AaBbCc (F1 trihybrid) with aabbcc (recessive parent).

These genes appear linked, based on the data, especially with phenotypes 3) and 6) showing very few recombinants (5/1700).Rough and normal (#1 95 , #2 50 phenotypes ) are also low.But Rough and variable have high frequecy too 700, as well as low frequency 5 .Rough and normal have a lower co-occurence frequency 95 #1 and #2.Green and variable has lower frequency..50 and 5 (#7 and #3).

Parental phenotypes are #8 (yellow,glossy,variables) and #1 (Green,rough,normal) and genotypes are aabbcc and AABBCC.

The actual configuration of the choromosomes of the parents ;

a/A...c/C......b/B

The double cross over progeny is represented by phenotypes #3 and #6