Below are data from a protein purification: Which purification step has the leas
ID: 276203 • Letter: B
Question
Below are data from a protein purification:
Which purification step has the least impact on target protein purity? Why?
Protein purification
Fraction volume(ml)
Total protein
(mg)
Activity
(units)
Specific activity (unit/mg)
Step1
1400
10000
100000
10
Step 2
280
3000
96000
32
Step 3
90
400
80000
200
Step 4
80
100
24000
240
Step 5
6
3
21000
7000
Protein purification
Fraction volume(ml)
Total protein
(mg)
Activity
(units)
Specific activity (unit/mg)
Step1
1400
10000
100000
10
Step 2
280
3000
96000
32
Step 3
90
400
80000
200
Step 4
80
100
24000
240
Step 5
6
3
21000
7000
Explanation / Answer
Answer:
Based on the given data:
The step that has the least impact on protein purity is the Step4. This is because there is no considerable or significant increase in the protein specific activity from Step 3 (200 units/mg) to Step 4 (240 units/mg).
In all other steps, there is a significant improvement in protein specific activity.
Purification Fraction volume(ml) Total protein (mg) Activity (units) Specific activity (unit/mg) Step1 1400 10000 100000 10 Step 2 280 3000 96000 32 Step 3 90 400 80000 200 Step 4 80 100 24000 240 Step 5 6 3 21000 7000Related Questions
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