Interactives. British and Metric Conversions. Abont thin Exercise 4: Identificat
ID: 276291 • Letter: I
Question
Interactives. British and Metric Conversions. Abont thin Exercise 4: Identification and Quantification of Organic Molecules shake-> #4: #5: 1% starch 1% glycogen shake-to unknown (general unknown c. Add 3 drops of l,KI solution to each test tube d. Record your observations and conclusions on the chart provided below c. Also, place a drop of I, KI solution on each of the following: 1) a wedge of potato 2) a corn kernel 3) a Pea stiof white breas and conc 4) a piece of white bread f. Record the observations and conclusions of part "e" on the chart provided below. Is the starclh localized in the corn and pea sceds? Test PubeSubs TestedObservations (edlor changesConcluion distilled H2O glucose starch glycogen general unknown potato com pea bread B. Lipids Sudan Dye Test Sudan dye (Sudan IV) is a red dye that is absorbed by lipids, turning it a very bright red. Other solutions may mix with the dye, showing a pink color, but they do not actually combine with it and exhibit the bright red of a positive test. In other words, the Sudan dye is a lipid soluble dye. Materials Sudan IV dye Glass slides Distilled water Unknown Procedure a. Place 3 clean glass slides on a white surface (paper towel) b. Place a drop of the following solutions on the slides, as indicated below: Slide #1: distilled water oil #2: #3: unknown (general unknown) c. Add a few grains of Sudan dye to each drop and stir carefully with toothpicks (use a separate tooth- pick for each drop).Explanation / Answer
Please find the answers below:
Answer A: All starches and sugars turn the iodine solution blue-black upon reaction. Since potato and corn contain large amount of starch, they will give positive test (iodine solution turning blue) whereas pea and bread would not.
Answer B: All lipids bind to the Sudan dye and turn it bright red in color. Since oil only contains large amount of fats/lipids among all the samples, it will give a positive test whereas all other samples would keep the dye unchanged.
Answer C: According to the Biuret test, presence of a peptide causes intercalation of cuprous ions in it thus giving a bluish color to the solution. Thus, among the samples, only milk and bovide serum albumin will give a positive Biuret test with appearance of blue color since they contain proteins.
Answer D: According to the nucleic acid test, the test reagent binds with the nucleic acids to give blue color. Thus, only the DNA or RNA containing tubes will give a positive reaction. However, the water used for experimentation is generally free of contaminations from nucleic acids and thus will give no result.
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