ecampus.ww.edu Question Completion Status O Some amino acids are coded by multip
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ecampus.ww.edu Question Completion Status O Some amino acids are coded by multiple codons. QUESTION 2 1pointsSv The four proteins mentioned above assist in eukaryotic transcription. Which of the following is in eukaryotes ?TBP (TATA bindirg proton in TF1) TATA box O-10/35 B QUESTION 3 1 points Save A Transoriotion factors assist RINA polymerase binding. This is necessary because RNA polymerase has DNA entry and exit channels RNA polymerase has a nucleotide entry channel ORNA polymerase is promiscuous- it will transcibe wherever a bieds RNA polymerase is able to break hydrogen bonds, but not phosphociester bonds Biology Chegg tutors MacBook Air 3 5 7 8Explanation / Answer
Q2) Which is a trans-regulatory element in Eukaryotes.
Answer- A- TBP (TATA binding protein in TFIID)
Explanation- Trans-regulatory elements are those elements, or proteins, which bind to the acting sequences to control gene expression.
cis-properties of different trans-acting factors are -
They are subunits of RNA polymerase
They bind to RNA polymerase to stabilize the initiation complex
They bind to all promoters at specific sequences but not to RNA polymerase (TFIID factor which binds to the TATA box)
They bind to a few promoters and are required for transcription initiation; these are positive regulators of gene expression.
So TBP (TATA binding protein in TFIID is an example of Trans-regulatory element.
TATA box is a cis-regulatory element.
Sigma is a protein required for initiation of transcription. It is a bacterial transcription initiation factor which helps in specific binding of RNA.
-10 and -35 are bacterial or prokaryotic promoters, they help in transcription process of prokaryotes, in binding or RNA polymerase to this promoter region. The -10 and the -35 elements are named so because they come from 35 and 10 nucleotides before the initiation site (+1 in the DNA). The minus sign indicates that they are before, not after, the initiation site.
Q3) Transcription factor assists RNA polymerase binding. This is necessary because --
Answer- C- RNA polymerase is promiscuous, it will transcribe wherever it binds.
Explanation-
During transcription process RNA polymerase helps in transcription that is it helps in copying DNA into RNA. But RNA polymerase cannot distinguish the site where to start transcription, it can get attached anywhere, but Transcription factors helps RNA polymerase in recognizing the specific DNA sequences or promoters region of the gene, where it gets attached. So basically, the promoter or the transcription initiation factors tells the polymerase where to get attached on the DNA and start transcription. Each gene has specific promoter.
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