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History Bookmarks Window Help i ecampus .ww.edu Question Completion Staus QUESTION 10 3 poir The last question is a short answer/essay. You will have to think about what you know from class and put your ideas into words. An experiment was conducted in which sections of a cukaryote's DNA were deleted to see the impact on mRNA production. In each case, transcriptionwas measared by looking at the number of transcripts produced after the gene expression was induced. Given the data below, explain why each of the deletions has the impact it does (or does not) (3-4 sentences are probably necessary for a complete answer Region deleted (all regions are upstream of the transcripAmount of mRNA produced (# of transcripts produced in start site No deletion 1500 through-1200 1200 through-900 500 through-350 350 to-100 10 minutes after gene induction) 15000 1000 15000 15000 1000 100 t0-1 otes Comments MacBook Air 88814 ?" 2 3 4

Explanation / Answer

No deletion: It has no effect on transcription.

-1500 through -1200: This region contains distal enhancers which are the regulatory trasncription factor binding sites. Enhancers may decrease or increase the rate of transcription. Here, we observe decreased rate of transcription.

-1200 through -900: No regulatory sites are present in this region.

-500 through -350: No regulatory sites are present in this region.

-350 through -100: This region contains proximal promoter elements which are the regulatory sites where specific trasncription factors can bind and change the transcription of a gene. Thus we observe decreased level of trasncription rather than complete shut down.

-100 through -1: This region belongs to the core promoter region where RNA polymerase actually binds and start the transcription. Deletion in this region may result in complete silencing of the gene.

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