OVO-Kit # 243 Ion Exchange Chromatography udy Questions Answer the following stu

Question

OVO-Kit # 243 Ion Exchange Chromatography udy Questions Answer the following study questions in your laboratory a separate worksheet orebook or ona 1. What is the basis for the separation of different compoundhs toy ion ex- change? How can molecules with the same charge at varying amounts be vepa- rated by chromatography? 2. 3. Why are celluloses often used as supports to separate large biologically 4. What do you think would happen if 0.5M K: acetate were used first to 5. Why is it important to prepare a standard curve for each spectropho- active proteins? elute the sample? Why? tometer?

Explanation / Answer

Ans 1 :

The biomolecule purification is usually done using Ion exchange technique. It basically uses the idea of interaction of oppositely charged molecules.

The sample to be purified contains charged molecules which is then passed over stationary phase containing oppositely charged molecules.

So the seperation of molecules take place with respect to their charges.

Related Questions

Navigate

• Browse (All)
• Browse O
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.