Lab 6 2. 3-GAATTCCCCCC-5 5. 5-TAAGAAGATACAG-3 PROBLEM 1: Nucleic Acids Draw in s

Question

Lab 6 2. 3-GAATTCCCCCC-5 5. 5-TAAGAAGATACAG-3 PROBLEM 1: Nucleic Acids Draw in schematic form the nucleotide GTP (a) Point out the 5' end and the 3' end. This nucleotide is RNA? How can you tell? What would it be called if it was DNA? (b) Draw out the reaction for the addition of a second base, adenosine, to this nucleotide. (c) What was the energy source for the reaction you drew in B? PROBLEM 3: DNA REPLICATION Make a diagram of the DNA replication fork Point out where the following things are ikely located (1) the leading strand. (2) the lagging streo (3) RINA primers (4) Helicase () primase (6) Okazak fragments ()Ligase (8) DNA polymerase (9) DNA Polymerase I (10) the 5 and 3' ends (d) Why is it that guanosine base-pairs with oytosine and not thymineJadenosine or (e) Why is the G-C basepait stronger than the A-T basepar0 PROBLEM 2: More DNA. You have & DNA strand with the following linear sequence of bases: 5-TGCGCCTG&AATICAG-3; (8) What is the sequence of the complementary strand that can base-pair with this particular DNA strand? (b) Here is another strand of DNA How is this strand different from the one in Problem 2A and what is the sequence of the omplementary strand? (c) Here are a bunch more DNAs. Draw the base paired complementary strands. Note the 5 and 3 ends 1. 5-GGCCTTAAAA-3 4.3-CCTTTAGGCCCTTT-5 rds English (us)

Explanation / Answer

Problem 2

a) Complementary sequence is

3' A C G C G G A C T T T A A G T C 5'

b) The DNA has polaity, the growing end of DNA is 3' and the other end is 5'. The polarities of the two strands are different.

5' A C G C G G A C T T T A A G T C 3'

c)

1. 3' CCGGAATTTTT 5'

2. 5' CTTAAGGGGGG 3'

3. 3' GGAAATCCGGGAAA 5'

4. 5' GGAAATCCGGGAAA 3'

5. 3' ATTCTTCTATGTC 5'

6. 5' TTTGGGCCCAAA 3'

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