please answer in detail if applicable. thank you! Name: Section: Date: to BMS 50
ID: 277673 • Letter: P
Question
please answer in detail if applicable. thank you!
Name: Section: Date: to BMS 504 General Microbiology Laboratory Laboratory 15 Questions - Genetic transfer by conjugation 1. Explain why you would not expect any growth on the agar plates containing only the donor or recipient cells. r What specific genes were transferred from donor to recipient cells Write the phenotype of the recombinant cells (Hint: look at the phenotype of the K57 and AlW2 strains in the Materials section) Briefly describe the mechanism of conjugation 2. 3. 4.Explanation / Answer
1. When donor and recipient are left on agar expected not growth because then they are in conjugation and link is formed via sex pili and areanot free to divide pr grow.
2. tra genes are transferred from donor to recipient.
3. Phenotype of the recombinant are different from wild type and can be grown on selective media. This will only alllw to grow recombinant. Different phenltype based selection strategy area like blue white screening with LacZ alpha complimentation etc.
4. Cells are made competent for the donor DNA. And a recombination bridge is formed between hfr and F- cells. tra genes are transferred to recipient and cells are transformed. F- cells become F+.
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