Digestion of DNA With A Restriction Endonuclease You need to digest 150ng of you

Question

Digestion of DNA With A Restriction Endonuclease You need to digest 150ng of your plasmid DNA with EcoRI. The maximum volume of your sample that you can load on the agarose gel is 40?. Your DNA sample is 25?g/ml. You want to use 8 Units of BamHI, which is provided at a concentration of 2 Unit/ul in 1X Reaction Buffer. The Gel Sample Buffer is a 5X stock. Keep this in mind since it must be included in your 40ul final volume. To assemble this reaction, you would combine the following: 40 ql a ul DNA 1.2ul water ul 10X Reaction Buffer Jul EcoRI restriction enzyme L? ul 5X Gel Sample Buffer After 60 minutes at 37°C, add Need Ison asst?× 100ng = a5000 ng/ mL

Explanation / Answer

Answer:

** The description mentions to use EcoRI for digestion, but sample details for BamHI are provided.

Solution 1: BamHI enzyme (provided in 1x Reaction buffer):

Solution2: DNA sample preparation (to be diluted in final 1x Reaction buffer):

Solution 3: Sample buffer 5x to working concentration 1x

Final Mix:

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