1. a) Pulse field gel electrophoresis (PFGE) is capable of separating extremely
ID: 278498 • Letter: 1
Question
1. a) Pulse field gel electrophoresis (PFGE) is capable of separating extremely large pieces of DNA by size through angled and switching electrical currents. It can clearly separate smal chromosomes, something that regular gel electrophoresis can't di. In a certain diploid plant, the chromosomes are small but differ from each other in size. Te plant has 2N· 56 chromosomes. If the chromosomal DNA is carefully extracted and analyzed by PFGE, how many DNA bands should be visible? b) A tetraploid plant has 18 different size chromosomes. If you did a Karyotype, how many s would you find? c) A woman has a chromosomal abnormality trisomy for the X chromosome. How many Barr bodies would you expect to typically find in her cells? d) Humans are diploid organism with 23 different chromosomes. A woman with Turner syndrome has one X chromosome and no Y. How many chromosomes would you find in a karyotype of this child? 2. A man who is sex-linked recessive colorblind marries a woman who carries sex-linked recessive factor VIII hemophilia. What genetic event or events could cause each of the following? (be very specific about any meiotic divisions involved, and parent of origin) a) Daughter with Turner syndrome, colorblind. b) Klinefelter syndrome son with hemophilia c) Klinefelter syndrome son who is colorblind in one eyeExplanation / Answer
1] One chromosome consist of one DNA molecule or one strand then, 56 chromosome will form (56 DNA) on pulse field gel electrophoresis which will be visible.
B) Karyotyping is arrangement of chromosome based on their shape, size, so, if there are 18 different kind or type of chromosome then (thenkaryotype is 18)
c) The women with trisomy x will have (2 Barr bodies) in their genome.
d) The karyotype of the child will be(X0) it will be a female child
2)
a) Turner syndrome is not inherited but caused due to defect in meiosis during the formation of gametes which can cause the girl to have xo sex chromosome a type of defect in gametogenesis.
The daughter will be colour blind since the father will pass a defective x chromosome to the girl during segregation of gametes or meiosis.
b) In klienfeliter syndrome an extra pair of sex chromosome or (X chromosome) is passed to the boy from either of his parents.
If the mother gives the defective X chromosome during gamete formation of meiosis then the boy can have this condition.
c) In klienfeliter syndrome an extra pair of sex chromosome or (X chromosome) is passed to the boy from either of his parents
The colour blindness in one eye is never genetic it could due to damage to optic nerve, ciliary muscle .sclera, cornea, convex lens in eyes region.
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.