Question 8 [14 marks /14 minutes] Design mutations of hypothetical protein-codin

Question

Question 8 [14 marks /14 minutes] Design mutations of hypothetical protein-coding genes a) Design a null mutation where the site of the mutation is outside the Open Reading Frame (ORF). Explain how your mutation fits the definition of a null mutation What type of mutagen could generate your null mutation? Explain. Design a mutation that is not a null allele. Explain the consequence of your mutation for the [5 marks] b) 2 marks] c) function of the protein. [5 marks] d) What type of mutagen could generate your second mutation? Explain. 12 marks]

Explanation / Answer

null mutation is a change in gene sequence so that it will either produce a non-functional protein or no protein production.

upstream of any ORF either insertion or deletion of a nucleotide will alter the sequence of the ORF. this will shift the ORF and it will end up with non-functional protein or no protein synthesis. ethidium bromide and proflavine are the intercalating agents which cause a frameshift mutation.

non null allele means functional gene. this is the mutation which does not alter the ORF of a gene. the gene product will be normal and functional.this mutation includes silent mutation, missense mutation (upstream of ORF).

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