4. The following is a code for a strand of DNA. DNA 3, A T A T TTT 1 2 3 4 5 6 7

Question

4. The following is a code for a strand of DNA. DNA 3, A T A T TTT 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 U G A C G U mRNA tRNA Amino Acid ATAT = Promoter sequence a. Using the genetic code provided in Figure 8.8, fill in the blanks U G G Met to complete the segment of DNA shown. b. Fill in the blanks to complete the sequence of amino acids coded for by this strand of DNA. c. Write the code for the complementary strand of DNA com pleted in part (a). d. What would be the effect if C were substituted for T at base 10? e. What would be the effect if A were substituted for G at base 11?

Explanation / Answer

a. 3’ATAT TACTTTGCATCCACT 5’

b. mRNA : AUG AAA CGU AGG UGA

Met Lys Arg Arg Stop

c.complementary strand will be

TATAATGAAACGTAGGTGA

d.If T is substituted by the C then the reading frame will be changed and the resulting frame will be 3’ATAT TACTTCGCATCCACT 5’

and the resulting mRNA will be

AUG AAU CGU AGG UGA

Met Asp Arg Arg Stop

e. If A is replaced by G at base 11 then the DNA strand will be

3’ATAT TACTTTACATCCACT 5’

then the resulting mRNA will be

mRNA : AUG AAA UGU AGG UGA

and the amino acid will be different

Met Lys Cys Arg Stop

f. If G is substituted at base 14

DNA will be 3’ATAT TACTTTGCAGCCACT 5’

mRNA : AUG AAA CGU CGG UGA

Amino acid: Met Lys Arg Pro Stop

g. If C is inserted between 9 and 10

then DNA strand will be

3’ATAT TACTTCTGCATCCACT 5’

mRNA will be AUG AAG ACG UAG GUG A

amino acids will be Met Lys Thr Stop

h. UV radiation will form TT dimer as there are consecutive three T in the sequence and there will be halt in transcription and no product will be formed.

i. Nonsense sequence is UGA at place 17,18,19

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