Question2 A dehydrogenase enzyme was designed de novo using a maquette protein i

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Question2 A dehydrogenase enzyme was designed de novo using a maquette protein into which had been substituted a binding site for the redox cofactor, FMN. It was predicted that the resulting flavoprotein would be able to use NADH and molecular oxygen to carry out the following oxidation of methane to methanol NADHHO2CH4NAD H20CH3OH Both cofactors used in this enzymatic reaction show characteristic absorption spectra as indicated in the figures below 2.0 1.5 NAD 0.8 1.0 0.2 0.5 0.0 220 240 260 280 300 320 340 360 380 Wavelength Inm) 0.0 500 600 WAVELENGTH (nm) Fig. 2. UV-visible spectrum of FMN in its oxidized and reduced form. How would you test the maquette to see if it oxidized methane using NADH and FMN via absorbance spectra? (how would you test if the maquette worked?)

Explanation / Answer

To test if the mequette is working we will perform the test and take one control which will not undergo the oxidation. Then we will take the spectral readings of both the control and the reaction test. The test which has NADH is in its reduced form will give a maximum absorption at 280nm while the product of the reaction test will give a max absorption at 340nm due to the oxidized form of NAD.

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