Question 10 15 marks 15 minutesl Indicate the level of expression of the lac ope
ID: 280042 • Letter: Q
Question
Question 10 15 marks 15 minutesl Indicate the level of expression of the lac operon in the various mutants shown in the table below (only relevant parts of the genotype are shown). You must answer in your answer book No lactose (no glucose) none Lactose present Glucose present Low Lactose present No glucose high Escherichia coli genotype 1. wildtype 2. lacP (affects lac operon promoter) 3. lacl 4. lacl 5. lacl Slaco 6. lacl*/ lacl (partial diploid) 7. CAP gene 8. CAP gene lacoc 9. CAP gene- laci 10. CAP gene CAP gene (partial diploid)Explanation / Answer
E.Coli genotype
No lactose
No glucose
Lactose present
Glucose present
Lactose present
Glucose absent
Wild type
None
Low
High
Lac p-
none
Low
None
LacI-
None
High
High
LacIs-
None
Low
None
Lac Is Lac O-
None
None
None
LacI+ lac Is
none
None
none
Cap-
None
Low
High
Cap gene- lacIs
none
None
Low
CAP gene- LacOc
None
High
High
Cap gene+ CAP gene-
High
Low
high
E.Coli genotype
No lactose
No glucose
Lactose present
Glucose present
Lactose present
Glucose absent
Wild type
None
Low
High
Lac p-
none
Low
None
LacI-
None
High
High
LacIs-
None
Low
None
Lac Is Lac O-
None
None
None
LacI+ lac Is
none
None
none
Cap-
None
Low
High
Cap gene- lacIs
none
None
Low
CAP gene- LacOc
None
High
High
Cap gene+ CAP gene-
High
Low
high
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