the amount of EDTA needed to prepare 100 ml of 0.5 M EDTA pH 8.0. The EDTA MW-37

Question

the amount of EDTA needed to prepare 100 ml of 0.5 M EDTA pH 8.0. The EDTA MW-372.2 g/mole 4. As EDTA dissolves in water, the [H'l1 will increase (note that full name of EDTA includes "acetate"). To increase to a pH 8.0, would you need to add HCl or NaOH? Determine the amounts/volumes of the chemicals needed to prepare the following solutions: 5. 100 ml of 0.1 N HCl from concentrated (12.0 M) HCl [For HCI, 1 N = 1 MI 100 ml of 1.0 M Tris pH = 7.6. Mw of Tris base is 121 g/mole. 6, 7. pH 7.6 is close to a neutral pH. When making 1.0 M Tris base, would you expect to need HCl or NaOH to decrease the pH to 7.6? To do electrophoresis, you will need to make a solution called TAE, which is a specific mixture of Tris base, acetic acid, and EDTA. Assuming no errors and no spills, you will need a minimum of 50 ml 1X TAE to make your agarose gel plus 1 liter of IX TAE for gel running buffer. TAE is normally made as a 50X concentrated stock.

Explanation / Answer

5) The formula we use is M1V1 = M2V2 (M=molarity, V=volume)

We have to calculate Volume V1

So, 12M X V1 = 0.1M X 100ml

That gives 0.83 ml

Take 0.83 ml of 12MHCl and 99.17ml of water, that gives 100 ml of 0.1 N HCl solution

6) 121 gms of Tris in 1000ml water gives 1M solution

In order to make 100ml of 1M Tris solution, add 12.1 gms of Tris in 100ml water

7) 1.0 M Tris base solution is a basic solution, which pH would be around 11

In order to decrease the pH to 7.6, we have to add HCl (acidic solution)

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