6. You are interviewing for a job in a research lab and the professor who runs t
ID: 280167 • Letter: 6
Question
6. You are interviewing for a job in a research lab and the professor who runs the lab asks if you are familiar with microarray experiments. You want the job, so you say, "yes. Unfortunately he/she then asks you to explain the steps involved starting with cells grown under two different conditions, Please explain in detail how one processes the samples to prepare them for application to the microarray slide, what the different spots already present on the microarray are, and ) what the possible results are for the different spots on the microarray.Explanation / Answer
1. While preparing the samples for microarray experiments, one shoulld take care of the following-
2. there are thousands of spots already present in the microarray slide, these spots contains a known gene or DNA sequence.
3. The experimental cDNA and reference cDNA, are tagged with red and green flourescent dye respectively,then mixed together and allowed to bind to the microarray slide.
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.