10. Here are the result of a qRT-PCR when looking at the expression levels of a

Question

10. Here are the result of a qRT-PCR when looking at the expression levels of a frog developmental gene called third-eye (3rdE) when tadpoles are grown in water containing the presence vs. absence of bisphenol A (a common pollutant from plastics). The researchers used RPO21 as their reference gene. Conditions Values Bisphenol A Clean water 3rdE 16 RPO21 14 12 1. Under which condition is the third-eye gene more highly expressed? How many times more highly expressed is third-eye in the condition you mentioned compared to the other condition? 2.

Explanation / Answer

ans-1 according to the data given in the table the third eye gene is highly expressed when bisphenol A is present along with water as compared to the clean water.

ans-2 the gene is expressed as much times= 16-11 =5

= 5/11 *100 = 45%

so the gene is expressed 45% more times as compared to clean water.

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