probes-a 13. The diagram below shows Pre-mRNA for YFG along with three probes th

Question

probes-a 13. The diagram below shows Pre-mRNA for YFG along with three probes that were designed for northern blot. Probe (a) binds to intron 1. Probe (b) is a "split probe* in which the first half of the probe binds to the end of exon 2 and the last half of the probe binds to the beginning of exon 3. Probe 19.Q .0 (c) binds to exon 3. The sizes of the introns and exons are indicated. On the three northern blots to the right, draw bands at the proper sizes where you'd expect to find bands using the different probes. 4.0 3.0 2.0 1.5 1.0 Exon 1Exon2Exon 3 Intron 1 Intron 2 1.5 kb 1 kb 1 kb 1.5 kb 1 kb

Explanation / Answer

Answer

In all the three columns (A B C) the band will appear at position 6. Because the lenght of the Pre-mRNA is 6 kb

Question say Northern blotting of Pre-mRNA, not of mRNA, indicating both exons and introns are linked.

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