will prepare a serial dilution of a glucose stock solution. Notice that the gluc
ID: 280242 • Letter: W
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will prepare a serial dilution of a glucose stock solution. Notice that the glucose concentrations in this lab are described as "milligrams per deciliter (mg/dL) instead of molarity. This is because mg/dL is the typical unit used in medicine to measure glucose concentration in human blood. Your Turn In the lab, you will prepare the following serial dilution: You will be given a glucose stock solution that has a concentration of 60 mg/dL. You need to make 1 mL and 0.94 mg/dL. Distilled water will be the solvent in these dilutions concentration. There is no need to convert these concentrations to molarities.) What is the dilution factor for this serial dilution? What is the V2 for this serial dilution? glucose concentrations:30 mg/dL, 15 mg/dL, 7.5 mg/dL, 3.75 mg/dL, 1.88 mg/dL mg/dL instead of molarity. This is just another way to express Calculate the Vi for this serial dilution. have a unit, be sure to include it. Show your calculations in the space below. If your answer should Transfer your answers to Part I of the Lab Procedures. After determining the enzyme activity at different substrate concentrations, you will determine the effect of temperature on enzyme activity. First, the glucose and enzyme in separate tubes will be equilibrated at 4°C (ice water),-22°C (room temperature), 37°C (body temperature) and 65°C (hot). Then the glucose and enzyme will be mixed in a cuvette and the Absorbance measured every 15 seconds for 2 minutes Your lab group will determine the enzyme activity at different pH values of the buffer solution. The glucose substrate will be diluted into one of four buffer solutions that has been adjusted to a predetermined pH. Each sample will be mixed with the enzyme in a cuvette and the Absorbance measured every 15 seconds for 2 minutes.Explanation / Answer
dilution factor is 2 (The dilution factor is equal to the final volume divided by the aliquot volume and here final volume is 1 ml and aliquot volume is 0.5 so that 1/0.5=2)
V2 for this serial dilution is 1because we want prepare the final volume of 1 ml
as we know V1C1=V2C2
therefore V1=V2C2/C1
in this problem(for 30mg/dL) V1=?, C1=60mg/dL, V2=1 ml and C2= 30mg/dL
V1=1*30/60=0.5mg/dL(this will same for other calculations too)
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