Please provide detailed understandings for 4 and 5. Thank you!! 4. What type of

Question

Please provide detailed understandings for 4 and 5. Thank you!!

4. What type of inhibitor (red line) is represented in the following Lineweaver-Burk graph? How do you know?

Do you need to extend the abscissa (X-axis) to the left?

5. DG°' indicates the change in the standard free energy as a reactant is converted to product (in kJ/mol). Based on what you know about these values, which reaction below is the most favorable? _____ Explain your answer.

4. What type of inhibitor (red line) is represented in the following Lineweaver-Burk graph? How do you know? Do you need to extend the abscissa (X-axis) to the left? 2.5 2.0 n 1.5 ? 1.0 0.5 0 0 0.2 0.4 0.6 0.8 1.0 1.2 1/ISl (M-1) 5. AG" indicates the change in the standard free energy as a reactant is converted to product (in kJ/mol). Based on what you know about these values, which reaction below is the most favorable? AGo +30.50 AGo-0.391 G?' = +1.265 ?Go' -2,878 AGo -61.76 Explain your answer. a) ADP +PiATP b) Glucose-1-phosphate glucose-6-phosphate c) Glucose + fructose sucrose + H2O d) Glucose CO2+ H20 e) NADH + H+-NAD"

Explanation / Answer

4. Competitive inhibitor. The inhibitor does not change the Vmax (inverse of the value at both the lines intersecting at Y-axis), however it will change the Km value. Yes, we need to extend the X-axis to the left to obtain the Km and apparant Km (due to inhibitor) values.

5. e) will be the most favourable since it have the lowest delta G°' value (-61.76). Lower the free energy value, higher will be the stability of the product, hence more favourable be the reaction.

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