6) Plasmids are important in biotechnology because they are a) a vehicle for the
ID: 281004 • Letter: 6
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6) Plasmids are important in biotechnology because they are a) a vehicle for the insertion of recombination DNA into bacteria. b) recognition sites on recombinant DNA. c) surfaces from protein synthesis in eukaryotic recombinants. d) surfaces for respiratory proccsses in bacteria. 7) What is the genetic function of a restriction enzyme? a) It adds nucleotides to the growing strand of DNA. b) It joins nucleotides during replication. c) It joins nuclcotides during transcription. d) It cleaves nucleic acids at specific sites. e) It repairs breaks in sugar-phosphate backbones. 8) The restriction enzyme us sequences and plasmid DNA acts by ed in constructing h id molecules of certain gene a) opening DNA molecules at specific sites, leaving sticky ends exposed. b) sealing plasmid DNA and foreign DNA into a closed circle c) transcribing plasmid DNA into a transformed molecule d) allowing a hybrid plasmid DNA into a transformed molecule e) binding human genes to bacterial plasmids. 9) How does a bacterial cell protect its own DNA from restriction enzymes? a) By adding methyl groups to adenine and cytosine b) Using DNA ligase to seal the bacterial DNA into a closed circle c) Adding histones to protect the double-stranded DNA. d) By foming "sticky ends" of bacterial DNA to prevent the enzyme from attaching e) By reinforcing bacterial DNA structure with covalent phosphodiester bonds Use the following key to answer question 10 Ke I) Transform bacteria with recombinant DNA molccule II Cut the plasmid DNA using restriction enzymes. III) Extract plasmid DNA from bacterial cells. IV) Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments. V) Use DNA ligase to seal plasmid DNA to nonplasmid DNA. 10) From the list above, which of the following is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium? c) III, II·IV, V, I d) III IV. V.L II e) IV. V. , II, III 11) What is a major source of restriction enzymes? a) Plant cells b) DNA technology c) animal cells d) bacteria.Explanation / Answer
6) The answer is a.As the desired segment of DNA is inserted into the plasmid which is a extrachromosomal DNA in bacteria.It replicates and make multiple copies of it.
7) The answer is d.The site where it cuts it called restriction site.
8) The answer is a.As restriction enzyme cuts the DNA at specific restriction site.Then it cuts the plasmid and the intrested segment is inserted into the plasmid which is ligated by ligase.Then it is transformed into the bacteria.
9) The answer is a.Methylation causes the modificatio of DNA due to which recognition site couldn't be identified.
10) The answer is c.
11) The answer is d (bacteria).
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