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Crocodiles kill their prey by immobilizing them and holding them under water unt

ID: 281182 • Letter: C

Question

Crocodiles kill their prey by immobilizing them and holding them under water until they drown.

1. Look at the O2 binding curves in the presence and absence of CO2 for crocodile and human hemoglobin in panel A. How do crocodiles manage to hold their breath for over an hour?

Look at panel b, does the decreased O2 binding arise from difference between the human and crocodiles alpha subunits or beta subunits?

How many amino acids must be change in the human hemoglobin to give human hemoglobin a similar response to CO2?

How similar are the sequences of the two subunits in human and crocodile hemog

G 1 a The oxyen-binding curves of recombimant crocode H lorcies) and human Hb lsquarese determined the aosence ierney syentots; and peserce miled symbols of 5% con ?. The oxygenording quaes) determined in the absence tempty) and presence ed tions c, The onygen-binding curve of Hb Scuba. which s human Hb weh th@ Leu 34a Os. Ser 35g-Ala, Phe 36.-Tyr. Tr 37?-Gtn. Lys 31 ?-Met, mr 38»-Lys, Gin 3op-Arg, Phe 418-Tyr mutations, n the absence lempty oircle) and presence (ed circie) of CO. The oxygen binding curves of Hb Scuba lacking the Leu 100aPhe His 103a+Gin mutations were determined in the absence (empty square) and presence (tilled square) at Co METHODS. Synthetic genes encoding the a- and gobins of the Nie crocodie (C niotiusi Hb were each constructed by assembling 14 o A subsequent mutations were introduced by casnette were produced essentially as described in ref. 17. Nile crocodile andautomatic recording apparatus All the measuremets were made at ai dimaenc-s were expressed under the control of either tac or pH 7.4 r' 50 m-M b&Trs; with 0.1 M dione ors at 25 C, ether ? lactac tandem promoter·Prntation of these recombinant bes was te pesence or absence of 5% ?, which??eu tram "e as described!?0ygen equilibrum curves were attained using ??? 21 mit bicarbonate iananthe buffer

Explanation / Answer

Answer 1.hemoglibin the iron pigment carries oxygen from lungs to the body. Crocodile use waste product of metabolism carbin di oxide dissolve in water and forms bicarbonate ion and triggers the hemoglobin to upload the oxygen. More energy the cells burn more oxygen is retired. But the efficient system of oxygen allocation , combining with very slow metabolism states how it is able to survive under water.

Alpha subunits are responsible.

At least twelve amino acids replacement is required to get similar response to co2.

The sequence of crocodiles and human hemoglobin is 98% similar.

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