Which of the following is mismatched concerning microscopy. A) SEM: 3D represent

Question

Which of the following is mismatched concerning microscopy.

A) SEM: 3D representation of surface features of cells and other objects, individual components in microbial communities, microbial environment interactions

B) Confocal: uses laser light sources to scan differentially labeled specimens producing #D-like images assembled by a computer.

C) Darkfield: can visualize living spirochetes in wet mounts, motility of cells in wet mounts.

D) Differential Interference Phase Contrast: similar to phase contrast but adds prisms providing additional detail and quasi-3D effect

E) Limit of resolution human eye (conventional light microscopy): 0.2mm (2nm)

Please explain.

Explanation / Answer

The B is mismatched concerning microscopy. The confocal uses puntual ilumination with a pinhole to eliminate information that is outside the focal plane. This happens because only the light that is inside this plane can be detected. It requires a scanning to have bi o tri dimentional images as only iluminates one point at a time.

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