culture is diluted 1:10 before counting Y squares; the formula then reads Y = #

Question

culture is diluted 1:10 before counting Y squares; the formula then reads Y = # counted x 25 x 50 x 1000 x 10. Part B Dry Weight Determination PROCEDURE Weight of a cell: A Petroff-Hausser Counting Chamber was used to adjust a suspension of cells of Escherichia coli to 1010 cells/ml. The suspension was then centrifuged and washed to remove any solutes, dried at 100 C overnight and the residue weighed. The dry weight of 10t0 E. coli cells was determined to be 5 mg. Given this number, calculate the weight of an individual cell in grams. Show your work in your lab notebook under Exercise 5. 41

Explanation / Answer

Answer: 0.5 pg

Explanation:

Total number of cells = 10^10

Total mass = 5 mg

Mass of individual cell = 5 mg/10^10 = 5 X 10^-10 mg = 5 X 10^-13 g = 0.5 pg

The average mass of an E coli cell is in the range of 0.5 to 1 pg

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