Question 5 of 19 Incorrect Map Lehninger Principles of Biochemistry Nelson Cox M

Question

Question 5 of 19 Incorrect Map Lehninger Principles of Biochemistry Nelson Cox MHE/Freeman by Sapling Learning OX SEVENTH EDITION Mixtures of amino acids can be analyzed by separating the mixture into its components through ion- exchange chromatography. Amino acids placed on a cation-exchange resin containing sulfonate (-SO3) groups flow down the column at different rates because of two factors that influence their movement: (1) ionic attraction between the sulfonate groups on the column and positively charged functional groups on the amino acids, and (2) hydrophobic interactions between amino acid side chains and the strongly hydrophobic backbone of the polystyrene resin For each pair of amino acids listed, determine the order of elution from a cation exchanger in pH 7.0 buffer. Amino Acid Pair Elutes First Asp and Arg Lys and Leu Gly and Glu Ala and Met Trp and Ser Asp Leu Glu Met Ser Elutes Second Arg Lys Gly Trp Incorrect One or more of the amino acids is placed incorrectly. Met is more hydrophobic than Ala and will interact more strongly with the hydrophobic polystyrene resin Previous ? Give Up & View Solution Try Again ? Next Exit

Explanation / Answer

Ans.

Amino acid pair Elutes first Elutes second Asp and Arg Asp Arg Arg is positively charged Lys and Leu Leu Lys Lys is positively charged Gly and Glu Gly Glu Glu is more hydrophobic Ala and Met Ala Met Met is more hydrophobic Trp and Ser Ser Trp Trp is more hydrophobic

Related Questions

Navigate

• Browse (All)
• Browse Q
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.