Review Questions What i? the difference between the lytic cycle and the lysogeni

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Review Questions What i? the difference between the lytic cycle and the lysogenic cycle of bacteriophages? 1. ie cycle: Vira) DNA enters te cll transcribes sel into the hos, cen messenger RNAs uses them to direot we bosoes lySogenic aycle: The DNA Or RNA enters the o integrates Ho Ahe hosA DNA as a reuw sed ef genes 2. How does a plaque form in a lawn of bacteria cells? wwnen lysis occur s sis:disintegration aby roptue tho cell wall hmemibrare 3. The process of using a known bacteriophage to identify an unknown bacterium is called? e phage typing 4. Why is a phage specific for certain types of bacteria? 5. You set up a serial dilution by transferring 0.5 ml of stock into Tube A with 4.5 ml of dilution. This solution is mixed and 1 ml of Tube A is transferred to Tube B with 9 ml of diluent. A 3d dilution is set up by transferring 0.5 from Tube B into 4.5 ml of diluent in Tube C.What is the total dilution from this serial dilution? 6. Suppose you wanted to isolate a virus that infects Bacillus subtils, a bacterium which normally inhabits soils. Describe how you would have to modify the procedure used in this lab to do that. Do you think phages can be used to successfully treat infectious diseases like Cholera or Tapeworms? 7. t cculd be an alterative te ontibiotics oire ogents f antimicrobial action yes, Haa aganst bacter ia 244 Bacteriophages: Reviewl Exercise 33

Explanation / Answer

4. When a phage infect a bacteria, the choice of bacteria mainly depends on presence ofreceptors which are specific to specific type of bacteria on the bacterial cell surface, which phage virus can detect and attach. Thus a phage can only bind selectively to the particular bacteria carrying the specific receptor.

5.dilution of tube A is 0.5ml in 4.5 ml ( 0.5/0.5+4.5= 0.5/5) or 1/10

when 1 ml of tube A is transfered to tube B with 9ml soluion, the dilution is then 1/10 X 1/10= 1/100

now when 0.5 ml from tube B is transferred to tube C, then the final dilution is 1/100 X 0.5/5 =1/100 X 1/10 =1/1000

6. here to isolate the virus, we require to isolate the bacteria Bacillus subtilis from the soil. As virus requires a host to grow, culture of partcular bacteria can used to isolate them. Now, to isolate the bacteria, soil can be first mixed with distilled water and then diluted serially. Next, particular amount of diluted solution is plated on agar plate. Bacterial colonies will grow on the plate depending on dilution and a pure culture of Bacillus subtilis with the specific virus present can be isolated.

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