ANSWER ALL QUESTIONS COMPLETELY To perform APOE gene testing as project 3 in our

Question

ANSWER ALL QUESTIONS COMPLETELY

To perform APOE gene testing as project 3 in our genetics lab session, which of the techniques below is not required? 1. a. b. c. d. DNA isolation Restriction enzyme digestion Gel electrophoresis DNA sequencing Restriction Fragment Length Polymorphism (RFLP) is a difference in homologous DNA sequences that can be detected by the presence of fragments o different lengths. These fragments are obtained by digestion of the DNA samples in question with specific__. 2. a. Proteinase K b. Restriction endonucleases

Explanation / Answer

Answer:

1. (d) DNA sequencing

Explanation:

The APOE gene encodes a protein called Apolipoprotein E. The APOE protein is a type of protein that is responsible for packaging cholesterol and other fats.

The testing of APOE gene involves DNA isolation, restriction digestion of the isolated DNA and running the samples in gel electrophoresis.

2. (b) Restriction endonucleases

Explanation:

Proteinase K exhibits broad substrate specificity and degrades many proteins in the native state.

In case of RFLP, restriction endonucleases are used which specifically cleaves DNA.

3. (c) DNA or protein

Explanation:

Polyacrylamide gels typically provide much smaller pores than do agarose gels and is formed by the radical polymerisation of acrylamide monomers.

Polyacrylamide gels can be used for the separation and analysis of proteins and relatively small nucleic acid molecules.

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