1. What is the average size of fragment a random sequence enzyme that has a 6-ba

Question

1. What is the average size of fragment a random sequence enzyme that has a 6-base recognition site? A. 6 bp 8. 24 bp C. 256 bp D. 1296 bp E. 4098 bp 2. What is the approximate number of fragments made if 16kb DNA is da enzyme that has a 6-base recognition site? A. 1 fragment B. 2 fragments C. 4 fragments D. 6 fragments E. 12 fragments result. The height of For 3-5: You performed a Sanger sequencing reaction and obtained the following the peaks is unimportant. The 5 end of the sequence is at the left of the trace. 3. Would the sequence of the primer, which is not shown, be located to the left or to the right of this trace? A. To the left To the right 4. What is the sequence of the smallest DNA molecule that contains dideoxyC (ddC) in the sequencing eaction? (ignore the primer.) C. 3' AAC 5 D. 3' AACA 5

Explanation / Answer

1) The average size of fragments in a random sequence of DNA that has 6 base recognition site is,

= 4^6

= 4×4×4×4×4×4

= 4096 bp

(option E)

2) If 16 kb DNA is digested with a restriction enzyme that has 6 base recognition site, the approximate number of fragments made is,

4^x = 16

x = 2

(option B)

Related Questions

Navigate

• Browse (All)
• Browse 1
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.