A colleague discovered a gene that is mutated in late-stage(aggressive) cervical

Question

A colleague discovered a gene that is mutated in late-stage(aggressive) cervical tumors. He gives you a vector containing a wildtype copy of this gene that you transfect into your original cervical cancer cell line. You observe no difference in growth, cell death or any other properties in standard tissue culture tests.

You inject these transfected cells, as well as the original cancer cell line(not trasfected)into different groups of mice. You observe..

Primary tumors of similar size develop in both groups of mice

The original cancer cell line kills all the mice within 2 month of transfection and dissection of these mice show tumors throughout body

The transfected cancer cell line does not kill the mice within the 6 months of transfection

Which hypotheses are suggested by these observations?

The gene codes for a protein that...

1)inhibits cell division

2)promotes cell division

3)prevents apoptosis

4)prevents metastasis

5)repair DNA

6)promotes angiogenesis

Explanation / Answer

Two groups of mice were tested for expression of cancer genes. One group of mice were injected with original cancer cell line for cervical tumor and the other group of mice were injected with transfected cells of aggressive cervical tumors. Primary tumors of similar size developed in both the groups. The original cancer cell lines kill all the mice within 2 months and tumor cells were seen throughout the body. The transfected cell line does not kill the mice within 6 months of transfection. The hypothesis which support the results are that the metastasis is prevented in transfected cell lines (option 4).

Related Questions

Navigate

• Browse (All)
• Browse A
• Subjects

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.