1) a.The results from a Northern blot of rat muscle RNA probed with human dystro
ID: 28682 • Letter: 1
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1) a.The results from a Northern blot of rat muscle RNA probed with human dystrophin DNA show two RNAs instead of the one seen in mice RNA. There are two hypotheses to explain this information. One is that there are two genes for dystrophin in rats. What would be the easiest way to test this hypothesis? b. Another hypothesis to explain your dystrophin results is that there are two forms of the RNA made from a single gene and that they differ because of splicing differences. How might you test this hypothesis? Assume that the dystrophin gene can be "subcloned" so that you have small pieces in different clones. (This is very important.) c. You find that there are two genes for dystrophin in rat and hypothesize that both are active in muscle because both contain the same enhancer-activating region. How would you test this hypothesis? What kind of clones do you need, and what would you do to find similar regulatory regions in both? d. After much work, you find your hypothesis is correct; the two dystrophin genes in the rat share a common enhancer. One of your colleagues, working on the regulation of another muscle gene, is studying a protein she thinks may bind to your enhancers. How can you test her hypothesis? Assume she can isolate the protein. e. Once again, the hypothesis turns out to be correct. Your colleague's graduate student has cloned the gene for the enhancer-binding protein, and you decide to see if you can isolate the human equivalent. He will give you the clone and tell you its sequence. What experiments can you do? f. I am studying a protein sequence that I believe to be highly conserved through evolution. Using NCBI databases, how can I determine if my theory is correct?Explanation / Answer
You can begin by asking yourself, What can I do that will give me one result if my hypothesis is true and a different result if my hypothesis is false? Once you have found a subject for an experiment, you can design the experiment to find out whether or not your predictions were correct. For example, suppose the printer on your computer stops working. You form a hypothesis that there is something wrong with the wire connecting the printer to the computer. If your hypothesis were true, what would the results be? One result would be that if you replaced the wire with a working wire, the printer would work again. Using this idea, you could perform an experiment by borrowing a friend’s printer wire and hooking it up in place of your own. b) Making Predictions For each hypothesis, ask yourself what would be true if the hypothesis were true. For example, if the hypothesis about the wind were true, then it would also be true that the plant would not fall off the sill if the wind were blocked. c)yes we can determine because Database that groups biomedical literature, small molecules, and sequence data in terms of biological relationships.
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