We wish to check if two RFLP markers in corn are linked. RFLP1 can be found in 4

Question

We wish to check if two RFLP markers in corn are linked. RFLP1 can be found in 4,000 bp and 5,000 bp lengths while RFLP2 can be found in a 3,000 bp and 6,000 bp. A homozygote 4,000 bp and 3,000 bp, was crossed with a homozygote 5,000 bp and 6,000 bp. The F1 offspring was then crossed to a homozygote containing the 4,000 bp and 3,000 bp fragments. The following results were obtained: RFLP combinations Number of offspring 6,000 bp, 5,000 bp, 4,000 bp, 3,000bp 50 6,000 bp, 5,000 bp 48 6,000 bp, 5,000bp, 3,000bp 25 6,000 bp, 5,000bp, 4,000bp 24 Describe the data diagrammatically. If the two markers are linked, what is the map distance between the two.

Explanation / Answer

RISTRICTION FRAGMENT LENGTH POLYMORPHISM=RFLP

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