how to plot a michaelis-menton plot with a data table? where velocity is defined
ID: 29600 • Letter: H
Question
how to plot a michaelis-menton plot with a data table?
where velocity is defined as the number of pmol (picomoles: 1 x 10-12 moles) of product produced per second in the enzyme assay. Generate Michaelis Menten plots for each of the datasets above (i.e. one graph for each substrate/product combination). Make sure that you label the axes correctly. Generate Lineweaver Burk plots for each of the datasets above (i.e. one graph for each substrate/product combination). Make sure that you label the axes correctly and provide a table of data that you derived from the data above to generate the Lineweaver Burk plot. Use the Lineweaver Burk plots that you have generated to determine Km and Vmax for each substrate and report them in the form of a table. Which of the three substrates is best in terms of Km that the enzyme exhibits for it? Which of the three substrates is best in terms of the Vmax that the enzyme exhibits with it?Explanation / Answer
Plot: horizontal axis- substrate concentration vertical axis- velocity Km(michaelis-menten constant)- its the part of graph which represents substrate concentration(here approx near 25 uM) at which half the maximum velocity is attained(here 145pmol/sec) ie where these two points meet.
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