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Mouse fibroblast actin can be purified by sodium dodecyl sulfate gel electrophor

ID: 299963 • Letter: M

Question

Mouse fibroblast actin can be purified by sodium dodecyl sulfate gel electrophoresis and then used as antigen to obtain an anti-actin antibody in rabbits. This antibody can be used in indirect immunofluorenscence to visualize actin-containing filaments is a variety of cell types.

Intermediate filaments are complex helical structures composed of proteins that have a single a-helical domain interrupted by four spacer segments. They contribute to the structure and stability of mammalian cells even though they can change their arrangement depending on the motile state of the cell.

A feasible interpretation for the chemi-osmotic model of photophosphorylation is that illumination ultimately causes for pumping of protons into the thylakoid lumen and that the inequality of the proton gradient is sufficient to drive the regeneration of ATP

Please state if they're True or False. If they're FALSE, state where it's false and correct it please!

Thanks!

Explanation / Answer

Mouse fibroblast actin can be purified by sodium dodecyl sulfate gel electrophoresis and then used as antigen to obtain an anti-actin antibody in rabbits. This antibody can be used in indirect immunofluorenscence to visualize actin-containing filaments is a variety of cell types. True

Intermediate filaments are complex helical structures composed of proteins that have a single a-helical domain interrupted by four spacer segments. They contribute to the structure and stability of mammalian cells even though they can change their arrangement depending on the motile state of the cell.False

Intermediate filaments are complex helical structures composed of proteins that have a single a-helical domain that is made up of four alpha helical segments, separated by linker segments. They contribute to the structure and stability of mammalian cells but they cannot change their arrangement depending on the motile state of the cell as they play no role in cell motility.

A feasible interpretation for the chemi-osmotic model of photophosphorylation is that illumination ultimately causes for pumping of protons into the thylakoid lumen and that the inequality of the proton gradient is sufficient to drive the regeneration of ATP. True

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