3. Case Study A 60 year-old woman was recently diagnosed with chronic myelogenou

Question

3. Case Study A 60 year-old woman was recently diagnosed with chronic myelogenous leukemia (CML). Her physician requested a t(9:22) translocation test. Cytogenetic analysis of 20 metaphases derived from culture of her peripheral blood was reported as 46, Xx, t(9:22) Molecular analysis of the t(9:22) translocation was performed by reverse transcriptase-PCR using Forward primers that are complementary to the major and minor breakpoints in the BCR gene on chromosome 22 Reverse primer complementary to the c-abl gene on chromosome 9 Forward and reverse primers complementary to c-abl were included in the reaction mixture to serve as an RNA integrity (amplification) control o o o After reverse transcriptase -PCR, the products were separated by gel electrophoresis, and bands were visualized by ethidium bromide staining. PCR product was seen for The c-abl amplification control in the lane containing patient specimen t(9:22) translocation utilizing the b3a2 breakpoints What is the interpretation of these results? PCR product was seen in the patient lane, the size consistent with a product derived from a a) b) Why do we use an internal RNA integrity control? c) Are these results consistent? d) Is additional laboratory testing warranted to confirm the diagnosis of CML?

Explanation / Answer

a)

2)

3.

4.

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