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You are presented with a gram of a newly isolated animal virus. Electron microsc

ID: 30187 • Letter: Y

Question

You are presented with a gram of a newly isolated animal virus. Electron microscopy reveals the presence of a typical membrane surrounding the virus, and chemical analysis shows the presence of two membrane lipids, phosphatidylethanolamine and phosphatidylserine, as well as several membrane-associated proteins. You need to 1) determine which proteins are peripheral, which traverse the membrane, and which are located solely inside the cell, 2) determine if the lipids are symmetrically disposed in the two faces of the bilayer. Come up with a method using these two reagents to answer your question. (Reagents: EA, and IEA)

Explanation / Answer

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