Q6. This is a picture of a micropipette, what is the function of this in bacteri
ID: 302605 • Letter: Q
Question
Q6. This is a picture of a micropipette, what is the function of this in bacterial transformation? Q 7 A student has forgotten which plasmid she used in her E. coli transformation. It could have been plasmid containing resistance for kanamycin, ampicillin, or tetracycline resistant plasmid.. She decides to make up a special set of plates to determine the type the antibiotic resistance. The plates below show the results of the test. Plate I. LB agar with kanamycin Plate II. LB agar with ampicillin Plate LB agar with tetracycline Plate IV. LB agar 4 Which antibiotic are the bacteria resistant to?Explanation / Answer
6QUESTION ANS:
FUNCTION OF MICROPIPPETE IN BACTERIAL TRANSFORMATION
TRANSFORMATION DEFINITION:TRANSFER OF GENES FROM ONE BACTERIA TO ANOTHER BACTERIA BY NAKED DNA ( FRAGMENT OF DNA AFTER LYSIS OF BACTERIA )
1. IT IS USED TO ADD 250 ML OF ICECOLD CALCIUM CHLORIDE TO EACH OF THE TUBES.THEN PUT THE PIPPETE CAREFULLY BACK INTO THE PACKAGE IT CAME FROM,BEING CAREFUL NOT TO TOUCH THE TIP ON ANYTHING BUT INSIDE OF THE PACKAGE ,IT SHOULD STAY STERILE.
2. IT IS USED TO MIX THE BACTERIA COMPLETELY BY SUCKING THE SUSPENSION IN AND OUT OF THE TUBE TO MAKE NO CLUMPS OF BACTERIA LEFT IN THE TUBE.THEN PLACE THE PIPPETE BACK INTO ITS WRAPPER.
3.USED TO ADDING LIQUID BROTH SOLUTION TO TUBES'
4.USED TO TRANSFER SUSPENSION FROM TUBE TO PLATE.
7th QUESTION ANSWER
THE STUDENT HAD DONE THE ANTIBIOTIC SENSITIVITY TEST BY DISC DIFFUSION METHOD ( KIRBY-BAUER )
IN PLATE 1 AND PLATE 2 BACTERIA IS GROWN FREELY,AND IN THE PLATE 3 CLEAR ZONES CALLED " ZONE OF INHIBITIONS" ARE SEEN'
THERE FORE THE BACTERIA E.COLI HAS RESISTANCE TO KANAMYCIN AND AMPICILLIN AND SENSITIVE TO TETRACYCLINE .
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