1) The specificity of an exoglycosidase (enzyme that cleaves the non-reducing re

Question

1) The specificity of an exoglycosidase (enzyme that cleaves the non-reducing residue of a polysaccharide chain) was tested on several substrates. The structure of the substrate (a to i) is shown below. Note that: G = N-acetyl glucosamine, M = mannose, and Gr = reduced Nacetylglucosamine.

One mole of N-actylglucosamine is released after treatment of a, b, g or h with this enzyme. Two moles of N-actylglucosamine are released after treatment of molecules e and f. The enzyme does not modify molecules c, d, and i. Determine the specificity of the enzyme. Be as precise as possible. (6pts)

2) How many moles of N-actylglucosamine are released after treatment of molecules j and k? (2 pts)

Explanation / Answer

The enzyme n- acetylglucosami e has a substrate specificity .hence it acts acts only when mannose 6 phosphate is .Present.hence in c mannose 6 phosphate is not present and hence the reaction us not affected by this enzyme.

3 moles will be obtained from j and k as the mannose 6 phosphate used is higher as compared to used in all other reaction. 3 molecules of mannose 6 phosphate give 2 moles so 4 molecules will give 2.6 moles which can approximately be taken as 3 .so the answer is 3 moles.

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